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Ion pair reagents are essential as being a mobile-section additive when structurally or chemically or polarity clever inseparable carefully associated compounds are to be divided [21, 22]. One example is, if a combination of ionic and nonionic analyte(s) having the exact same polarity and identical retention time is necessary to become separated, g

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Analyte molecules partition between a liquid stationary stage along with the eluent. Equally as in hydrophilic conversation chromatography (HILIC; a sub-procedure within just HPLC), this process separates analytes based upon dissimilarities of their polarity. HILIC most frequently uses a bonded polar stationary section along with a mobile section d